Influence of calcium and magnesium on the secondary structure in solutions of individual caseins and binary casein mixtures

The influence of Ca and Mg addition on the secondary structure ?S1-, ?S2-, ?- ?-CN in solutions individual binary mixtures caseins was investigated using FTIR spectroscopy. Both their mixtures, resulted increase ?-sheet structures decrease triple helices turns, implying binding cations to similar sites. Binding with phosphoseryl clusters loop-helix-loop motifs explained reduction helical element. In addition, electronegative regions reduced electrostatic repulsion, resulting an hydrophobic interactions accounting for sheet structures. Compared Mg, it seemed that had more affinity caseins, especially when they were a mixture. information presented here expands present understanding casein interactions.